Info
Assistant Professor in the Department of Bioengineering and Therapeutic Sciences at UCSF and the Institute for Human Genetics. His laboratory focuses on building biophysical, molecular, and cellular understanding of membrane proteins using scalable technologies. Developed DIMPLE (deep insertion, deletion, and missense mutation libraries) for systematically generating and screening protein variants at scale. PhD in Daniel Schmidt’s lab at the University of Minnesota, developing massively parallel sequencing-based methods to study and engineer membrane proteins.
DiffUSE relevance
Coyote-Maestas’s deep mutational scanning and insertional scanning methods generate large variant libraries whose structural and functional effects are quantified at scale. Diffuse scattering from crystals of key variants could reveal how specific mutations perturb the conformational landscape. He is at UCSF, the same institution as Jaime Fraser (DiffUSE PI), Yifan Cheng, and Dr. Andrej Sali, making cross-lab collaboration easy.
Key Relationships
- UCSF — same institution as Jaime Fraser, Yifan Cheng, Dr. Andrej Sali
- DIMPLE — deep mutational scanning and insertional scanning methods
- Variant Effect Alliance (varianteffect.org) — listed member
- Membrane protein dynamics community
Sources
- Coyote-Maestas Lab: https://www.wcoyotelab.com/
- UCSF profile: https://pharmacy.ucsf.edu/willow-coyote-maestas
- DIMPLE paper (Coyote-Maestas et al., Genome Biology 2023): https://pubmed.ncbi.nlm.nih.gov/36829241/