Info
MolProbity is a web service for all-atom contact analysis and structure validation of proteins and nucleic acids. Developed by the Richardson Lab at Duke University, Department of Biochemistry. Operational since 2002. Four MolProbity criteria are integrated into the wwPDB validation “slider” on PDB entry pages. Core validation tools are also integrated into PHENIX.
People
| Name | Role | Affiliation |
|---|---|---|
| David Richardson | Co-PI | Duke University |
| Jane Richardson | Co-PI | Duke University |
| Christopher Williams | Developer | Duke University |
| Vincent Chen | Key contributor | Duke University (historical) |
| Mark Prisant | Developer | Duke University |
Jane Richardson
Jane Richardson is a pioneering structural biologist known for creating the first ribbon diagrams of protein structure. The Richardsons are also co-PIs on PHENIX. This dual role connects MolProbity validation directly to the PHENIX refinement pipeline.
Funding
- NIH/NIGMS R01-GM073919 (historical)
- NIH/NIGMS R35-GM131883 (current MIRA grant to D.C. Richardson)
- Duke University bridge funding
Key Relationships
- Integrated into PHENIX (phenix.reduce, phenix.probe, validation GUI)
- Four MolProbity criteria appear on wwPDB PDB entry pages
- Interactive all-atom contact dots available in Coot
- Mirror site at University of Manchester
- Jane Richardson and David Richardson are co-PIs on PHENIX
- MolProbity clashscore is the standard metric for model quality across the field
Key Reference
Williams et al., Protein Science 27, 293-315 (2018) Prisant et al., Protein Science 29, 315-329 (2020)